|
KMID : 1094719980030020071
|
|
Biotechnology and Bioprocess Engineering
1998 Volume.3 No. 2 p.71 ~ p.77
|
|
|
Effective production of N-acetyl-β-D-glucosamine bySerratia marcescens using chitinaceous waste
|
|
Kim Kwang
Creagh A. Louise
Haynes Charles A.
|
|
|
Abstract
|
|
|
|
The strain ofSerratia marcescens QM B1466 produces selectively large amount of chitinolytic enzymes (about 1mg/L medium). Enzymatic hydrolysis of chitin to N-acetyl-β-D-glucosamine (NAG) was performed with a system consisting of two hydrolases (chitinase and chitobiase) produced by optimization of a microbial host consuming chitin particles. For the development of Large-scale biological process for the production of NAG from chitinaceous waste, the selection and optimization of a microbial host, particle size of chitin and pretreatment of chitin source were investigated. Also, the effect of crab/shrimp chitin sources and initial induction time using chitin as a sole carbon source on chitinase/chitobiase production and NAG production were examined. Crab-shell chitin(1.5%) treated by dilute acid and, ball-milled with a nominal diameter less than 250m gave the highest chitinase activity over a 7 days culture. Crude chitinase/chitobiase solution obtained in a 10 L fed-batch fermentation showed a maximum activities of 23.6 U/mL and 5.1 U/mL, respectively with a feeding time of 3 hrs, near pH 8.5 at 30°C.
|
|
|
KEYWORD
|
|
|
Serratia marcescens QM B1466, chitinase/chitobiase, N-acetyl-β-D-glucosamine chitinolytic hydrolysis, feeding strategy, enzyme induction, chitin pretreatment
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|